Human PARK7/DJ-1 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 5.36 ng/ml (50 μl)；9.83 ng/ml (10 μl)； |
| Standard Curve Range | 15.63~1000 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0645 | 0.0580 | 0.0613 | |
| 15.63 | 0.0995 | 0.0885 | 0.0940 | 0.0328 |
| 31.25 | 0.1349 | 0.1260 | 0.1305 | 0.0692 |
| 62.50 | 0.2386 | 0.2181 | 0.2284 | 0.1671 |
| 125.00 | 0.4859 | 0.4609 | 0.4734 | 0.4122 |
| 250.00 | 1.0050 | 0.9409 | 0.9730 | 0.9117 |
| 500.00 | 2.0200 | 1.8750 | 1.9475 | 1.8863 |
| 1000.00 | 3.6210 | 3.4590 | 3.5400 | 3.4788 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 25.6 | 98.7 | 300.9 | 30.2 | 89.7 | 298.6 |
| Standard Deviation | 7.1 | 15.4 | 57.9 | 8.2 | 15.5 | 69.4 |
| Coefficient of Variation(%) | 3.6 | 6.4 | 5.2 | 3.7 | 5.8 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PARK7/DJ-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 70% to 105% with an overall mean recovery of 85%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 51.93-342.23 | 100 | 153.14 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: PARK7/DJ-1
Park7/DJ-1 is one of several genes thought to cause autosomal-recessive early-onset parkinson's disease. It also acts as a positive regulator of androgen receptor-dependent transcription, as well as functions as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death.
