Human FOLR1 DTSet enzyme-linked immunoassay kit
| Specification | 96*5 Test；96T*15 Test |
|---|---|
| Standard Curve Range | 46.88 pg/ml -3000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Not Ready-to-Use |
| Test Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0172 | 0.0165 | 0.0169 | |
| 46.88 | 0.0579 | 0.0669 | 0.0624 | |
| 0.0456 | 93.75 | 0.1303 | 0.1231 | 0.1267 |
| 0.1099 | 187.50 | 0.2263 | 0.2564 | 0.2414 |
| 0.2245 | 375.00 | 0.5634 | 0.4782 | 0.5208 |
| 0.5040 | 750.00 | 1.1860 | 1.2520 | 1.2190 |
| 1.2022 | 1500.00 | 2.8620 | 2.9120 | 2.8870 |
| 2.8702 | 3000.00 | 4.3398 | 4.2738 | 4.3068 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DTSet Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: YOUKE Life, Catalog # DSEP01. Plate Sealers: YOUKE Life, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . YOUKE Life, Catalog # DSCB01.
- Blocking Buffer: YOUKE Life, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. YOUKE Life, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.YOUKE Life, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. YOUKE Life, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. YOUKE Life, Catalog # DSSS01.
Product Data Sheet
Background: FOLR1
Folate receptor α (FRα), also known as folate receptor 1 (FOLR1), belongs to the folate receptor family and are both cysteine-rich glycolipid anchor proteins. FRα is encoded by FOLR1 and is a cell surface glycoprotein with a molecular weight of 38-40KD. It was initially discovered as a folic acid-binding protein. It was later found that in addition to transporting folic acid, it is also involved in regulating the proliferation and metastasis of tumor cells. Folic acid is an important nutrient necessary for maintaining cell growth and metabolism, and it plays a key role in the synthesis of DNA and RNA.
