Human MICB enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.09 pg/ml (50 μl)；6.68 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0208 | 0.0166 | 0.0187 | |
| 6.86 | 0.0321 | 0.0300 | 0.0311 | 0.0124 |
| 20.58 | 0.0507 | 0.0474 | 0.0491 | 0.0304 |
| 61.73 | 0.0996 | 0.0959 | 0.0978 | 0.0791 |
| 185.19 | 0.2567 | 0.2492 | 0.2530 | 0.2343 |
| 555.56 | 0.7067 | 0.7088 | 0.7078 | 0.6891 |
| 1666.67 | 2.0300 | 1.9550 | 1.9925 | 1.9738 |
| 5000.00 | 3.8400 | 3.7960 | 3.8180 | 3.7993 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 104.3 | 521.4 | 1862.1 | 99.0 | 528.2 | 1622.6 |
| Standard Deviation | 4.2 | 23.5 | 101.7 | 8.2 | 28.2 | 62.3 |
| Coefficient of Variation(%) | 4.1 | 4.5 | 5.5 | 5.2 | 5.3 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MICB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from84% to 108% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.38-168.59 | 100 | 45.33 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD137/4-1BB/TNFRSF9 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: MICB
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response.
