Human B7-H6/NCR3LG1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.98 pg/ml (50 μl)；4.61 pg/ml (10 μl) |
| Standard Curve Range | 20.48~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0203 | 0.0208 | 0.0206 | |
| 20.48 | 0.0313 | 0.0305 | 0.0309 | 0.0104 |
| 51.20 | 0.0403 | 0.0421 | 0.0412 | 0.0207 |
| 128.00 | 0.0750 | 0.0767 | 0.0759 | 0.0553 |
| 320.00 | 0.1884 | 0.2017 | 0.1951 | 0.1745 |
| 800.00 | 0.6884 | 0.7111 | 0.6998 | 0.6792 |
| 2000.00 | 2.0969 | 2.2789 | 2.1879 | 2.1674 |
| 5000.00 | 4.0442 | 4.1880 | 4.1161 | 4.0956 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 111.1 | 559.2 | 1692.1 | 103.7 | 522.2 | 1594.1 |
| Standard Deviation | 6.7 | 35.9 | 40.2 | 7.8 | 25.2 | 62.4 |
| Coefficient of Variation(%) | 6.0 | 6.4 | 2.4 | 7.5 | 4.8 | 3.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human B7-H6 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 119% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 159.96-719.34 | 100 | 459.11 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of B7-H6 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: B7-H6/NCR3LG1
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis.
