Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.47 pg/ml (50 μl)；5.07 pg/ml (10 μl) |
| Standard Curve Range | 15.63~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0659 | 0.0698 | 0.0679 | |
| 15.63 | 0.1978 | 0.2148 | 0.2063 | 0.1385 |
| 31.25 | 0.3346 | 0.3512 | 0.3429 | 0.2751 |
| 62.50 | 0.5916 | 0.6533 | 0.6225 | 0.5546 |
| 125.00 | 1.1130 | 1.1470 | 1.1300 | 1.0622 |
| 250.00 | 1.8960 | 1.9670 | 1.9315 | 1.8637 |
| 500.00 | 3.2850 | 3.4530 | 3.3690 | 3.3012 |
| 1000.00 | 4.3356 | 4.3766 | 4.3561 | 4.2883 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 25.6 | 117.0 | 352.9 | 22.8 | 110.2 | 319.1 |
| Standard Deviation | 1.4 | 3.5 | 10.2 | 1.1 | 2.3 | 12.4 |
| Coefficient of Variation(%) | 5.3 | 3.0 | 2.9 | 4.7 | 2.1 | 3.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD137/4-1BB/TNFRSF9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 116% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 20.14-49.28 | 100 | 32.58 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD137/4-1BB/TNFRSF9 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD137/4-1BB/TNFRSF9
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the
TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.
