Human IL-3R alpha/CD123 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 22.75 pg/ml (50 μl)；129.58 pg/ml (10 μl) |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0491 | 0.0475 | 0.0483 | |
| 156.25 | 0.0991 | 0.0987 | 0.0989 | 0.0506 |
| 312.50 | 0.1488 | 0.1489 | 0.1489 | 0.1006 |
| 625.00 | 0.2471 | 0.2517 | 0.2494 | 0.2011 |
| 1250.00 | 0.4688 | 0.4276 | 0.4482 | 0.3999 |
| 2500.00 | 0.8168 | 0.8312 | 0.8240 | 0.7757 |
| 5000.00 | 1.5140 | 1.5180 | 1.5160 | 1.4677 |
| 10000.00 | 2.7410 | 2.7560 | 2.7485 | 2.7002 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 219.2 | 1152.6 | 3499.9 | 194.9 | 1053.1 | 3264.2 |
| Standard Deviation | 12.1 | 67.3 | 170.5 | 12.8 | 58.3 | 145.9 |
| Coefficient of Variation(%) | 5.5 | 5.8 | 4.9 | 6.5 | 5.5 | 4.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-3R alpha/CD123 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 95% with an overall mean recovery of 86%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 804.66-2639.86 | 100 | 1600.42 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-3R alpha/CD123 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-3R alpha/CD123
Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.
