Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.02 pg/ml (50 μl)；4.22 pg/ml (10 μl) |
| Standard Curve Range | 40.96~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0068 | 0.0081 | 0.0075 | |
| 40.96 | 0.0280 | 0.0282 | 0.0281 | 0.0207 |
| 102.40 | 0.0630 | 0.0590 | 0.0610 | 0.0536 |
| 256.00 | 0.1461 | 0.1605 | 0.1533 | 0.1459 |
| 640.00 | 0.4384 | 0.4124 | 0.4254 | 0.4180 |
| 1600.00 | 1.1440 | 1.1410 | 1.1425 | 1.1351 |
| 4000.00 | 2.9610 | 2.8740 | 2.9175 | 2.9101 |
| 10000.00 | 4.0079 | 4.1190 | 4.0635 | 4.0560 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 218.3 | 1133.2 | 3538.1 | 221.8 | 1122.4 | 3350.1 |
| Standard Deviation | 6.8 | 40.5 | 130.7 | 6.3 | 60.8 | 184.5 |
| Coefficient of Variation(%) | 3.1 | 3.6 | 3.7 | 2.8 | 5.4 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Oxytocin-neurophysin 1/OXT into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 119% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 23.53-1127.81 | 100 | 175.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Oxytocin-neurophysin 1/OXT in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Oxytocin-neurophysin 1/OXT
This gene encodes a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream.
