Human Endostatin/COL18A1 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.31 ng/ml (10 μl) |
| Standard Curve Range | 1.37~1000 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0066 | 0.0063 | 0.0065 | |
| 1.37 | 0.0117 | 0.0122 | 0.0120 | 0.0055 |
| 4.12 | 0.0255 | 0.0263 | 0.0259 | 0.0195 |
| 12.35 | 0.0680 | 0.0758 | 0.0719 | 0.0655 |
| 37.04 | 0.2051 | 0.2193 | 0.2122 | 0.2058 |
| 111.11 | 0.7228 | 0.6482 | 0.6855 | 0.6791 |
| 333.33 | 1.8520 | 1.8470 | 1.8495 | 1.8431 |
| 1000.00 | 2.8380 | 2.9900 | 2.9140 | 2.9076 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 18.6 | 90.3 | 323.1 | 25.8 | 108.2 | 374.8 |
| Standard Deviation | 0.8 | 3.7 | 11.3 | 1.1 | 4.7 | 18.7 |
| Coefficient of Variation(%) | 4.4 | 4.1 | 3.5 | 4.2 | 4.3 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Endostatin/COL18A1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 120% with an overall mean recovery of 112%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 58.87-119.93 | 100 | 76.55 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Endostatin/COL18A1
Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.
