Human CD63 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.24 pg/ml (50 μl)；6.71 pg/ml (10 μl)； |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0151 | 0.0128 | 0.0140 | |
| 6.86 | 0.0286 | 0.0280 | 0.0283 | 0.0144 |
| 20.58 | 0.0572 | 0.0582 | 0.0577 | 0.0438 |
| 61.73 | 0.1475 | 0.1458 | 0.1467 | 0.1327 |
| 185.19 | 0.4194 | 0.3984 | 0.4089 | 0.3950 |
| 555.56 | 1.2120 | 1.1120 | 1.1620 | 1.1481 |
| 1666.67 | 2.6950 | 2.5740 | 2.6345 | 2.6206 |
| 5000.00 | 3.8760 | 3.8910 | 3.8835 | 3.8696 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 336.6 | 1807.6 | 5114.7 | 325.3 | 1844.1 | 5419.5 |
| Standard Deviation | 10.2 | 76.5 | 268.5 | 11.8 | 98.5 | 124.0 |
| Coefficient of Variation(%) | 3.0 | 4.2 | 5.2 | 3.6 | 5.3 | 2.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD63 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 119% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 570.74-1262.25 | 100 | 787.53 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD63 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD63
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.
