Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50 μl)；0.08 ng/ml (10 μl)； |
| Standard Curve Range | 0.41~300 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0056 | 0.0054 | 0.0055 | |
| 0.41 | 0.0188 | 0.0196 | 0.0192 | 0.0137 |
| 1.23 | 0.0493 | 0.0501 | 0.0497 | 0.0442 |
| 3.70 | 0.1445 | 0.1453 | 0.1449 | 0.1394 |
| 11.11 | 0.4360 | 0.4285 | 0.4323 | 0.4268 |
| 33.33 | 1.2330 | 1.2430 | 1.2380 | 1.2325 |
| 100.00 | 3.2110 | 3.1820 | 3.1965 | 3.1910 |
| 300.00 | 4.2906 | 4.2706 | 4.2806 | 4.2751 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 7.6 | 38.8 | 148.5 | 6.5 | 33.6 | 102.7 |
| Standard Deviation | 0.3 | 1.8 | 2.7 | 0.4 | 2.0 | 2.5 |
| Coefficient of Variation(%) | 3.6 | 4.5 | 1.8 | 6.0 | 6.0 | 2.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD115/M-CSF R/CSF-1-R into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 99% with an overall mean recovery of 89%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 174.98-741.02 | 100 | 502.94 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD115/M-CSF R/CSF-1-R in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD115/M-CSF R/CSF-1-R
M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta. Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain. M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction. The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals. M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC. M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis. In the brain, microglia expressing increased
M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear.
