Human CCL13/MCP-4 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.03 pg/ml (50 μl)；0.18 pg/ml (10 μl)； |
| Standard Curve Range | 0.69~500 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0085 | 0.0090 | 0.0088 | |
| 0.69 | 0.0257 | 0.0247 | 0.0252 | 0.0165 |
| 2.06 | 0.0596 | 0.0624 | 0.0610 | 0.0523 |
| 6.17 | 0.1737 | 0.1756 | 0.1747 | 0.1659 |
| 18.52 | 0.5100 | 0.4768 | 0.4934 | 0.4847 |
| 55.56 | 1.3790 | 1.2690 | 1.3240 | 1.3153 |
| 166.67 | 3.0120 | 2.9570 | 2.9845 | 2.9758 |
| 500.00 | 4.2130 | 4.3809 | 4.2970 | 4.2882 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 11.6 | 60.1 | 194.9 | 10.7 | 53.7 | 177.4 |
| Standard Deviation | 0.4 | 1.9 | 6.6 | 0.6 | 2.3 | 9.1 |
| Coefficient of Variation(%) | 3.1 | 3.2 | 3.4 | 5.2 | 4.3 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL13/MCP-4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 22.30-140.00 | 100 | 75.86 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL13/MCP-4 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CCL13/MCP-4
Human MCP-4, also known as CCL13, encodes a 98 amino acid (aa) precursor protein with a 23 aa hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature MCP-4. Mature MCP-4 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins.
