Human APOC3/Apolipoprotein CⅢ enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.20 ng/ml (10 μl) |
| Standard Curve Range | 1.37~1000 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 90min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0048 | 0.0041 | 0.0045 | |
| 1.37 | 0.0055 | 0.0058 | 0.0057 | 0.0012 |
| 4.12 | 0.0093 | 0.0098 | 0.0096 | 0.0051 |
| 12.35 | 0.0178 | 0.0218 | 0.0198 | 0.0154 |
| 37.04 | 0.0527 | 0.0598 | 0.0563 | 0.0518 |
| 111.11 | 0.2111 | 0.2129 | 0.2120 | 0.2076 |
| 333.33 | 0.9572 | 1.0480 | 1.0026 | 0.9982 |
| 1000.00 | 2.8340 | 2.9620 | 2.8980 | 2.8936 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 16.0 | 95.3 | 288.4 | 15.0 | 90.4 | 299.6 |
| Standard Deviation | 1.0 | 4.5 | 22.8 | 0.5 | 5.0 | 25.2 |
| Coefficient of Variation(%) | 6.4 | 4.7 | 7.9 | 3.6 | 5.5 | 8.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human APOC3/Apolipoprotein CⅢ into health Human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 70% to 118% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (µg/ml) | Detectable (%) | Mean of Detectable (µg/ml) |
|---|---|---|---|---|
| Serum | 30 | 9.51-215.14 | 100 | 85.02 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: APOC3/Apolipoprotein CⅢ
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein antibody is derived from human plasma by density gradient ultracentrifugation and HPLC.
