Rat IL-5 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.14 pg/ml (50 μl)；0.69 pg/ml (10 μl) |
| Standard Curve Range | 0.69~500 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0146 | 0.0141 | 0.0144 | |
| 0.69 | 0.0223 | 0.0231 | 0.0227 | 0.0084 |
| 2.06 | 0.0396 | 0.0367 | 0.0382 | 0.0238 |
| 6.17 | 0.0784 | 0.0810 | 0.0797 | 0.0654 |
| 18.52 | 0.2175 | 0.2006 | 0.2091 | 0.1947 |
| 55.56 | 0.5625 | 0.5697 | 0.5661 | 0.5518 |
| 166.67 | 1.5290 | 1.5880 | 1.5585 | 1.5442 |
| 500.00 | 3.5670 | 3.6160 | 3.5915 | 3.5772 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 10.6 | 55.6 | 169.9 | 11.4 | 55.2 | 171.9 |
| Standard Deviation | 0.5 | 2.2 | 8.0 | 0.2 | 1.5 | 3.6 |
| Coefficient of Variation(%) | 4.3 | 4.0 | 4.7 | 1.5 | 2.8 | 2.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat IL-5 into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.06-0.69 | 100 | 0.33 |
Serum/Plasma – Thirty samples from apparently healthy rats were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-5
IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
