Rat ICAM-1 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.04 ng/ml (50 μl)；0.07 ng/ml (10 μl) |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0060 | 0.0064 | 0.0062 | |
| 0.14 | 0.0153 | 0.0153 | 0.0153 | 0.0091 |
| 0.41 | 0.0352 | 0.0335 | 0.0344 | 0.0282 |
| 1.23 | 0.0946 | 0.0942 | 0.0944 | 0.0882 |
| 3.70 | 0.2485 | 0.2398 | 0.2442 | 0.2380 |
| 11.11 | 0.6446 | 0.6379 | 0.6413 | 0.6351 |
| 33.33 | 1.5470 | 1.5320 | 1.5395 | 1.5333 |
| 100.00 | 2.7720 | 2.7450 | 2.7585 | 2.7523 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 4.3 | 17.2 | 45.8 | 4.5 | 17.8 | 48.5 |
| Standard Deviation | 0.1 | 0.3 | 1.7 | 0.1 | 0.4 | 0.9 |
| Coefficient of Variation(%) | 2.7 | 1.9 | 3.7 | 3.1 | 2.5 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat ICAM-1 into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 13.35-34.28 | 100 | 22.48 |
Serum/Plasma – Thirty samples from apparently healthy rats were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: ICAM-1
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.
