Mouse TREM2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.60 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0253 | 0.0312 | 0.0283 | |
| 2.74 | 0.0348 | 0.0328 | 0.0338 | 0.0056 |
| 8.23 | 0.0466 | 0.0418 | 0.0442 | 0.0160 |
| 24.69 | 0.0882 | 0.0877 | 0.0880 | 0.0597 |
| 74.07 | 0.1885 | 0.1928 | 0.1907 | 0.1624 |
| 222.22 | 0.5228 | 0.4881 | 0.5055 | 0.4772 |
| 666.67 | 1.3710 | 1.3660 | 1.3685 | 1.3403 |
| 2000.00 | 3.1040 | 3.1460 | 3.1250 | 3.0968 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 35.4 | 192.9 | 626.2 | 39.0 | 202.6 | 679.0 |
| Standard Deviation | 2.7 | 13.0 | 47.8 | 1.8 | 7.6 | 34.7 |
| Coefficient of Variation(%) | 7.6 | 6.7 | 7.6 | 4.5 | 3.8 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse TREM2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 107% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 7959.19-21290.31 | 100 | 14637.59 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: TREM2
TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types. It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC. A single human TREM2 gene has been described, however, two closely related orthologs were reported in mouse. The proteins differ by only three amino acids and were designated TREM2a and TREM2b. TREM2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail. It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals. Ligation of TREM2 on immature DC with anti-TREM2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts. In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM2 deficient individuals.
