Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 pg/ml (10 μl) |
| Standard Curve Range | 0.27~200 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0059 | 0.0063 | 0.0061 | |
| 0.27 | 0.0196 | 0.0204 | 0.0200 | 0.0139 |
| 0.82 | 0.0474 | 0.0530 | 0.0502 | 0.0441 |
| 2.47 | 0.1412 | 0.1520 | 0.1466 | 0.1405 |
| 7.41 | 0.4025 | 0.4208 | 0.4117 | 0.4056 |
| 22.22 | 1.1140 | 1.2170 | 1.1655 | 1.1594 |
| 66.67 | 2.8660 | 2.8730 | 2.8695 | 2.8634 |
| 200.00 | 4.1954 | 4.3502 | 4.2728 | 4.2667 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 5.4 | 26.4 | 74.1 | 4.0 | 21.4 | 79.3 |
| Standard Deviation | 0.4 | 2.1 | 4.3 | 0.2 | 0.4 | 4.1 |
| Coefficient of Variation(%) | 6.7 | 7.8 | 5.8 | 4.8 | 2.0 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Pro-collagen I alpha 1/COL1A1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 119% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 146.01-320.89 | 100 | 223.31 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Pro-collagen I alpha 1/COL1A1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Pro-collagen I alpha 1/COL1A1
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
