Mouse PD-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 7.65 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0264 | 0.0261 | 0.0263 | |
| 13.72 | 0.0304 | 0.0335 | 0.0320 | 0.0057 |
| 41.15 | 0.0453 | 0.0421 | 0.0437 | 0.0175 |
| 123.46 | 0.0771 | 0.0764 | 0.0768 | 0.0505 |
| 370.37 | 0.1637 | 0.1724 | 0.1681 | 0.1418 |
| 1111.11 | 0.4431 | 0.4195 | 0.4313 | 0.4051 |
| 3333.33 | 1.2060 | 1.2660 | 1.2360 | 1.2098 |
| 10000.00 | 3.2720 | 3.0420 | 3.1570 | 3.1308 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 187.2 | 963.7 | 3386.5 | 201.2 | 1007.7 | 3360.1 |
| Standard Deviation | 10.5 | 69.0 | 211.2 | 4.4 | 35.5 | 223.5 |
| Coefficient of Variation(%) | 5.6 | 7.2 | 6.2 | 2.2 | 3.5 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse PD-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 84% with an overall mean recovery of 87%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 133.17-389.78 | 100 | 261.5 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CRP in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: PD-1
PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Blockade of the PD-1: PD-L1 interaction using anti-PD-1 antibodies enhances understanding of anti-tumor immunity and advances the potential for cancer immunotherapy.
