Mouse IL-21 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.22 pg/ml (10 μl) |
| Standard Curve Range | 78.13~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0282 | 0.0270 | 0.0276 | |
| 78.13 | 0.0690 | 0.0779 | 0.0735 | 0.0459 |
| 156.25 | 0.1152 | 0.1219 | 0.1186 | 0.0910 |
| 312.50 | 0.2242 | 0.2356 | 0.2299 | 0.2023 |
| 625.00 | 0.4089 | 0.4331 | 0.4210 | 0.3934 |
| 1250.00 | 0.8430 | 0.8296 | 0.8363 | 0.8087 |
| 2500.00 | 1.6260 | 1.7010 | 1.6635 | 1.6359 |
| 5000.00 | 3.2850 | 3.2960 | 3.2905 | 3.2629 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 122.1 | 592.6 | 1834.3 | 106.1 | 524.2 | 1569.8 |
| Standard Deviation | 8.0 | 39.3 | 88.3 | 6.1 | 21.1 | 73.4 |
| Coefficient of Variation(%) | 6.6 | 6.6 | 4.8 | 5.8 | 4.0 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-21 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 96% with an overall mean recovery of 89%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-5.89 | 66.7 | 2.50 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-21 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-21
IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.
