Mouse IL-16 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.43 pg/ml (10 μl) |
| Standard Curve Range | 12.29~3000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0275 | 0.0264 | 0.0270 | |
| 12.29 | 0.0387 | 0.0434 | 0.0411 | 0.0141 |
| 30.72 | 0.0524 | 0.0630 | 0.0577 | 0.0308 |
| 76.80 | 0.0947 | 0.1025 | 0.0986 | 0.0717 |
| 192.00 | 0.1932 | 0.2175 | 0.2054 | 0.1784 |
| 480.00 | 0.4853 | 0.5141 | 0.4997 | 0.4728 |
| 1200.00 | 1.2560 | 1.3040 | 1.2800 | 1.2531 |
| 3000.00 | 3.1270 | 2.9260 | 3.0265 | 2.9996 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 1002.69 | 371.96 | 79.16 | 960.9 | 340.3 | 72.3 |
| Standard Deviation | 65.41 | 23.29 | 5.45 | 47.0 | 19.0 | 5.7 |
| Coefficient of Variation(%) | 6.5 | 6.3 | 6.9 | 4.9 | 5.6 | 7.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-16 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 119% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 259.31-1393.01 | 100 | 505.88 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-16
Interleukin 16 (IL-16), also named lymphocyte chemoattractant factor (LCF), is a 14-17 kDa single chain non-glycosylated polypeptide that was originally identified as a CD8+ T cell-derived chemoattractant for CD4+ cells. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. In addition to its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro.
