Mouse IL-12/IL-23 p40 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.65 pg/ml (10 μl) |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0227 | 0.0230 | 0.0229 | |
| 156.25 | 0.1802 | 0.1725 | 0.1764 | 0.1535 |
| 312.50 | 0.3242 | 0.3088 | 0.3165 | 0.2937 |
| 625.00 | 0.5863 | 0.5805 | 0.5834 | 0.5606 |
| 1250.00 | 1.1220 | 1.1500 | 1.1360 | 1.1132 |
| 2500.00 | 2.2430 | 1.9900 | 2.1165 | 2.0937 |
| 5000.00 | 3.5490 | 3.4890 | 3.5190 | 3.4962 |
| 10000.00 | 4.4060 | 4.5161 | 4.4611 | 4.4382 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 254.3 | 1384.4 | 3649.7 | 229.4 | 1118.2 | 3402.2 |
| Standard Deviation | 6.7 | 43.2 | 130.4 | 13.2 | 63.8 | 131.2 |
| Coefficient of Variation(%) | 2.6 | 3.1 | 3.6 | 5.8 | 5.7 | 3.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-12/IL-23 p40 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 662.49-1451.18 | 100 | 1102.18 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-12/IL-23 p40
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
