Mouse IFN alpha-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.65 pg/ml (10 μl) |
| Standard Curve Range | 78.13~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0255 | 0.0288 | 0.0272 | |
| 78.13 | 0.1023 | 0.1111 | 0.1067 | 0.0796 |
| 156.25 | 0.1886 | 0.1889 | 0.1888 | 0.1616 |
| 312.50 | 0.3705 | 0.3604 | 0.3655 | 0.3383 |
| 625.00 | 0.7418 | 0.7227 | 0.7323 | 0.7051 |
| 1250.00 | 1.4051 | 1.4527 | 1.4289 | 1.4018 |
| 2500.00 | 2.7303 | 2.5847 | 2.6575 | 2.6304 |
| 5000.00 | 4.0742 | 4.1814 | 4.1278 | 4.1007 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 87.84 | 491.57 | 1544.40 | 88.73 | 480.46 | 1517.12 |
| Standard Deviation | 4.99 | 18.68 | 66.80 | 2.53 | 15.37 | 57.77 |
| Coefficient of Variation(%) | 5.7 | 3.8 | 4.3 | 2.8 | 3.2 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IFN alpha-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 98% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-26.30 | 66.7 | 13.29 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IFN alpha-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IFN alpha-1
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
