Mouse Coagulation Factor XII enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.35 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0164 | 0.0142 | 0.0153 | |
| 13.72 | 0.0253 | 0.0224 | 0.0239 | 0.0086 |
| 41.15 | 0.0286 | 0.0276 | 0.0281 | 0.0128 |
| 123.46 | 0.0472 | 0.0489 | 0.0481 | 0.0328 |
| 370.37 | 0.1131 | 0.1289 | 0.1210 | 0.1057 |
| 1111.11 | 0.3938 | 0.4223 | 0.4081 | 0.3928 |
| 3333.33 | 1.3840 | 1.4040 | 1.3940 | 1.3787 |
| 10000.00 | 3.3980 | 3.3740 | 3.3860 | 3.3707 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 186.2 | 1021.9 | 3453.9 | 143.3 | 846.7 | 2856.7 |
| Standard Deviation | 5.7 | 43.2 | 230.7 | 6.7 | 56.7 | 113.3 |
| Coefficient of Variation(%) | 3.0 | 4.2 | 6.7 | 5.5 | 6.6 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Coagulation Factor XII into health Mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 106% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 13.00-60.31 | 100 | 33.76 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Coagulation Factor XII in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Coagulation Factor XII
Coagulation factor XII (F12) is a serum glycoprotein that plays multiple roles in initiating blood coagulation, fibrinolysis, and the production of bradykinin and angiotensin.
