Mouse CD68/SR-D1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.62 pg/ml (10 μl) |
| Standard Curve Range | 78.13~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0409 | 0.0415 | 0.0412 | |
| 78.13 | 0.1598 | 0.1527 | 0.1563 | 0.1151 |
| 156.25 | 0.2903 | 0.2588 | 0.2746 | 0.2334 |
| 312.50 | 0.5202 | 0.4834 | 0.5018 | 0.4606 |
| 625.00 | 0.9210 | 0.8673 | 0.8942 | 0.8530 |
| 1250.00 | 1.6190 | 1.6390 | 1.6290 | 1.5878 |
| 2500.00 | 2.9040 | 2.9550 | 2.9295 | 2.8883 |
| 5000.00 | 4.2213 | 4.1789 | 4.2001 | 4.1589 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 141.4 | 654.7 | 1898.7 | 129.9 | 593.6 | 1759.2 |
| Standard Deviation | 5.9 | 35.1 | 107.5 | 7.6 | 34.7 | 80.1 |
| Coefficient of Variation(%) | 4.2 | 5.4 | 5.7 | 5.8 | 5.8 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD68/SR-D1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1438.48-2557.23 | 100 | 1971.16 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-1 beta in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CD68/SR-D1
CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.
