Mouse CCL22/MDC enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.27 pg/ml (10 μl) |
| Standard Curve Range | 15.63~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120 min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0274 | 0.0274 | 0.0274 | |
| 15.63 | 0.1367 | 0.1379 | 0.1373 | 0.1099 |
| 31.25 | 0.2705 | 0.2405 | 0.2555 | 0.2281 |
| 62.50 | 0.5103 | 0.4645 | 0.4874 | 0.4600 |
| 125.00 | 0.8967 | 0.8830 | 0.8899 | 0.8625 |
| 250.00 | 1.6390 | 1.6130 | 1.6260 | 1.5986 |
| 500.00 | 2.6470 | 2.6060 | 2.6265 | 2.5991 |
| 1000.00 | 3.6450 | 3.6560 | 3.6505 | 3.6231 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 85.7 | 232.7 | 472.2 | 27.2 | 131.3 | 391.8 |
| Standard Deviation | 3.9 | 11.7 | 24.2 | 1.4 | 5.8 | 9.3 |
| Coefficient of Variation(%) | 4.5 | 5.0 | 5.1 | 5.2 | 4.5 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CCL22/MDC into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 107% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 100.18-214.77 | 100 | 166.50 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CCL22/MDC in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CCL22/MDC
Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.
