Mouse Angiotensinogen/AGT enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.18 ng/ml (10 μl) |
| Standard Curve Range | 4.10~1000 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60 min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0070 | 0.0058 | 0.0064 | |
| 4.10 | 0.0238 | 0.0241 | 0.0240 | 0.0176 |
| 10.24 | 0.0523 | 0.0545 | 0.0534 | 0.0470 |
| 25.60 | 0.1480 | 0.1288 | 0.1384 | 0.1320 |
| 64.00 | 0.3872 | 0.3845 | 0.3859 | 0.3795 |
| 160.00 | 0.9081 | 1.0140 | 0.9611 | 0.9547 |
| 400.00 | 2.2850 | 2.0880 | 2.1865 | 2.1801 |
| 1000.00 | 3.7610 | 3.6150 | 3.6880 | 3.6816 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 21.9 | 108.5 | 346.2 | 18.7 | 86.6 | 261.0 |
| Standard Deviation | 1.2 | 5.4 | 16.0 | 1.2 | 3.2 | 14.9 |
| Coefficient of Variation(%) | 5.7 | 5.0 | 4.6 | 6.6 | 3.7 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse Angiotensinogen/AGT into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.63-11.41 | 100 | 9.86 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Angiotensinogen/AGT in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Angiotensinogen/AGT
Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II. Ang II induces vascoconstriction and aldosterone release. Mature human angiotensinogen is 452 aa in length (aa 34-485). It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41). Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.
