Mouse alpha 1 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.57 ng/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0085 | 0.0087 | 0.0086 | |
| 13.72 | 0.0168 | 0.0182 | 0.0175 | 0.0089 |
| 41.15 | 0.0345 | 0.0348 | 0.0347 | 0.0261 |
| 123.46 | 0.0903 | 0.0873 | 0.0888 | 0.0802 |
| 370.37 | 0.2459 | 0.2427 | 0.2443 | 0.2357 |
| 1111.11 | 0.6791 | 0.6760 | 0.6776 | 0.6690 |
| 3333.33 | 1.7800 | 1.7870 | 1.7835 | 1.7749 |
| 10000.00 | 3.7750 | 3.8150 | 3.7950 | 3.7864 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 203.5 | 1135.4 | 4091.5 | 165.0 | 911.8 | 3156.6 |
| Standard Deviation | 15.0 | 70.0 | 273.9 | 8.7 | 57.5 | 117.3 |
| Coefficient of Variation(%) | 7.4 | 6.2 | 6.7 | 5.3 | 6.3 | 3.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse alpha 1 antitrypsin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 210.39-335.68 | 100 | 288.48 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of alpha 1 antitrypsin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: alpha 1 antitrypsin
Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors. As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma. The deficiency is caused by point mutations in naturally occurring serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe serpin A1 deficiency.
