Monkey CRP/C-Reactive Protein enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 4.86 ng/ml (50 μl) |
| Standard Curve Range | 6.25~200 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120 min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0529 | 0.0529 | 0.0529 | |
| 6.25 | 0.0751 | 0.0862 | 0.0807 | 0.0278 |
| 12.50 | 0.1193 | 0.1274 | 0.1234 | 0.0705 |
| 25.00 | 0.2251 | 0.2136 | 0.2194 | 0.1665 |
| 50.00 | 0.4499 | 0.4835 | 0.4667 | 0.4139 |
| 100.00 | 1.1600 | 1.0320 | 1.0960 | 1.0432 |
| 200.00 | 2.5790 | 2.4600 | 2.5195 | 2.4667 |
Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Monkey CRP/C-Reactive Protein into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 2 | 55.18-70.1 | 100 | 56.17 |
Serum/Plasma – Two samples from apparently healthy volunteers were evaluated for the presence of Monkey CRP/C-Reactive Protein in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CRP/C-Reactive Protein
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
