Human von Willebrand Factor enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.02 ng/ml (50 μl)；0.69 ng/ml (10 μl)； |
| Standard Curve Range | 4.69~300 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| 0.00 | 0.0064 | 0.0058 | 0.0061 | |
|---|---|---|---|---|
| 4.69 | 0.0354 | 0.0345 | 0.0350 | |
| 0.0293 | 9.38 | 0.0859 | 0.0789 | 0.0824 |
| 0.0798 | 18.75 | 0.2116 | 0.1990 | 0.2053 |
| 0.2055 | 37.50 | 0.5367 | 0.5317 | 0.5342 |
| 0.5306 | 75.00 | 1.3890 | 1.3180 | 1.3535 |
| 1.3829 | 150.00 | 2.5750 | 2.7090 | 2.6420 |
| 2.5689 | 300.00 | 3.6260 | 3.6480 | 3.6370 |
| 3.6199 | ||||
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 5.7 | 32.8 | 151.8 | 5.3 | 31.3 | 113.6 |
| Standard Deviation | 0.2 | 1.7 | 10.4 | 0.1 | 2.0 | 7.7 |
| Coefficient of Variation(%) | 2.7 | 5.2 | 6.8 | 2.6 | 6.4 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human von Willebrand Factor into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 126% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 10.53-1936.87 | 100 | 1056.08 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of von Willebrand Factor in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: von Willebrand Factor
Von Willebrand factor (VWF) is a large multimeric glycoprotein produced in endothelial cells and megakaryocytes and present in subendothelial matrix, blood plasma and platelets. VWF mediates adhesion and aggregation of platelets at sites of vascular injury, which are critical for both haemostasis and thrombosis.
