Human VEGFR2/KDR enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.54 pg/ml (50 μl)；9.53 pg/ml (10 μl)； |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0221 | 0.0195 | 0.0208 | |
| 6.86 | 0.0336 | 0.0305 | 0.0321 | 0.0113 |
| 20.58 | 0.0500 | 0.0446 | 0.0473 | 0.0265 |
| 61.73 | 0.1072 | 0.1073 | 0.1073 | 0.0865 |
| 185.19 | 0.2788 | 0.2483 | 0.2636 | 0.2428 |
| 555.56 | 0.7927 | 0.7507 | 0.7717 | 0.7509 |
| 1666.67 | 2.1021 | 2.0794 | 2.0908 | 2.0700 |
| 5000.00 | 4.1378 | 4.1878 | 4.1628 | 4.1420 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 121.47 | 525.25 | 1597.38 | 116.40 | 515.89 | 1636.98 |
| Standard Deviation | 6.01 | 32.20 | 84.55 | 1.94 | 15.10 | 85.29 |
| Coefficient of Variation(%) | 4.9 | 6.1 | 5.3 | 1.7 | 2.9 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human VEGFR2/KDR into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 110% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.28-24.70 | 100 | 9.93 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VEGFR2/KDR in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: VEGFR2/KDR
VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
