Human VE Cadherin enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50 μl)；0.12 ng/ml (10 μl) |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0176 | 0.0176 | 0.0176 | |
| 0.14 | 0.0228 | 0.0224 | 0.0226 | 0.0050 |
| 0.41 | 0.0323 | 0.0328 | 0.0326 | 0.0150 |
| 1.23 | 0.0583 | 0.0614 | 0.0599 | 0.0423 |
| 3.70 | 0.1408 | 0.1456 | 0.1432 | 0.1256 |
| 11.11 | 0.3962 | 0.3948 | 0.3955 | 0.3779 |
| 33.33 | 1.0930 | 1.1360 | 1.1145 | 1.0969 |
| 100.00 | 2.8490 | 2.8830 | 2.8660 | 2.8484 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 37.7 | 10.4 | 2.0 | 32.5 | 9.0 | 1.8 |
| Standard Deviation | 1.8 | 0.5 | 0.1 | 0.9 | 0.3 | 0.1 |
| Coefficient of Variation(%) | 4.9 | 4.7 | 3.5 | 2.7 | 3.3 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human VE Cadherin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 122% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.18 | 18.8 | 0.13 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VE Cadherin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: VE Cadherin
Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are
calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.
