Human VCAM-1/CD106 enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.04 ng/ml (50 μl)；0.08 ng/ml (10 μl)； |
| Standard Curve Range | 0.27~200 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0127 | 0.0105 | 0.0116 | |
| 0.27 | 0.0203 | 0.0185 | 0.0194 | 0.0078 |
| 0.82 | 0.0316 | 0.0326 | 0.0321 | 0.0205 |
| 2.47 | 0.0798 | 0.0779 | 0.0789 | 0.0673 |
| 7.41 | 0.2252 | 0.2061 | 0.2157 | 0.2041 |
| 22.22 | 0.5731 | 0.5630 | 0.5681 | 0.5565 |
| 66.67 | 1.6260 | 1.6200 | 1.6230 | 1.6114 |
| 200.00 | 3.7320 | 3.6370 | 3.6845 | 3.6729 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 4.3 | 19.6 | 53.8 | 4.0 | 21.2 | 57.8 |
| Standard Deviation | 0.3 | 0.7 | 3.2 | 0.1 | 0.7 | 1.1 |
| Coefficient of Variation(%) | 6.3 | 3.4 | 5.9 | 1.8 | 3.2 | 2.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human VCAM-1/CD106 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 108% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 668.12-1561.06 | 100 | 1006.35 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VCAM-1/CD106 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: VCAM-1/CD106
VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.
