Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.06 pg/ml (50 μl)；0.87 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0193 | 0.0180 | 0.0187 | |
| 1.37 | 0.0348 | 0.0335 | 0.0342 | 0.0155 |
| 4.12 | 0.0687 | 0.0626 | 0.0657 | 0.0470 |
| 12.35 | 0.1540 | 0.1598 | 0.1569 | 0.1383 |
| 37.04 | 0.4233 | 0.3886 | 0.4060 | 0.3873 |
| 111.11 | 1.1080 | 1.0290 | 1.0685 | 1.0499 |
| 333.33 | 2.4510 | 2.4130 | 2.4320 | 2.4134 |
| 1000.00 | 3.8060 | 3.8000 | 3.8030 | 3.7844 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 16.4 | 85.0 | 258.5 | 17.5 | 92.7 | 302.1 |
| Standard Deviation | 0.7 | 2.3 | 14.5 | 0.7 | 3.9 | 15.9 |
| Coefficient of Variation(%) | 4.4 | 2.7 | 5.6 | 3.7 | 4.2 | 5.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ULBP1/NKG2DL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 120% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.18-1.88 | 100 | 0.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ULBP1/NKG2DL1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: ULBP1/NKG2DL1
ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance.
