Human Total HSP60 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.22 pg/ml (50 μl)；4.45 pg/ml (10 μl) |
| Standard Curve Range | 4.10~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0310 | 0.0327 | 0.0319 | |
| 4.10 | 0.0436 | 0.0462 | 0.0449 | 0.0131 |
| 10.24 | 0.0678 | 0.0691 | 0.0685 | 0.0366 |
| 25.60 | 0.1196 | 0.1236 | 0.1216 | 0.0898 |
| 64.00 | 0.2535 | 0.2621 | 0.2578 | 0.2260 |
| 160.00 | 0.5522 | 0.5783 | 0.5653 | 0.5334 |
| 400.00 | 1.2460 | 1.2700 | 1.2580 | 1.2262 |
| 1000.00 | 2.5840 | 2.5540 | 2.5690 | 2.5372 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 26.6 | 99.0 | 277.0 | 27.8 | 101.2 | 284.9 |
| Standard Deviation | 2.3 | 5.6 | 21.7 | 1.8 | 5.3 | 14.5 |
| Coefficient of Variation(%) | 8.5 | 5.6 | 7.8 | 6.3 | 5.2 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Total HSP60 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 66% to 73% with an overall mean recovery of 69%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-20.90 | 50 | 8.98 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: HSP60
Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically upregulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat-shock or other cellular stress. Full length human HSP60 is 98% identical to mouse and rat HSP60.
