Human TNF RII/TNFRSF1B enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.27 pg/ml (50 μl)；5.77 pg/ml (10 μl) |
| Standard Curve Range | 5.49~4000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0125 | 0.0118 | 0.0122 | |
| 5.49 | 0.0234 | 0.0224 | 0.0229 | 0.0108 |
| 16.46 | 0.0440 | 0.0410 | 0.0425 | 0.0304 |
| 49.38 | 0.1080 | 0.1087 | 0.1084 | 0.0962 |
| 148.15 | 0.2952 | 0.2944 | 0.2948 | 0.2827 |
| 444.44 | 0.8058 | 0.8283 | 0.8171 | 0.8049 |
| 1333.33 | 1.9330 | 1.9890 | 1.9610 | 1.9489 |
| 4000.00 | 3.6260 | 3.6600 | 3.6430 | 3.6309 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 77.5 | 375.0 | 1222.5 | 75.9 | 372.9 | 1201.0 |
| Standard Deviation | 4.4 | 18.1 | 69.5 | 4.2 | 16.8 | 26.2 |
| Coefficient of Variation(%) | 5.7 | 4.8 | 5.7 | 5.5 | 4.5 | 2.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TNF RⅡ/TNFRSF1B into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 120% with an overall mean recovery of 115%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 24.09-59.68 | 100 | 34.98 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TNF RⅡ/TNFRSF1B in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: TNF RII/TNFRSF1B
TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.
