Human TNF R1/TNFRSF1A enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50 μl)；0.13 ng/ml (10 μl) |
| Standard Curve Range | 0.07~50 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0187 | 0.0199 | 0.0193 | |
| 0.07 | 0.0329 | 0.0316 | 0.0323 | 0.0130 |
| 0.21 | 0.0593 | 0.0605 | 0.0599 | 0.0406 |
| 0.62 | 0.1474 | 0.1490 | 0.1482 | 0.1289 |
| 1.85 | 0.3976 | 0.4073 | 0.4025 | 0.3832 |
| 5.56 | 1.0320 | 1.0090 | 1.0205 | 1.0012 |
| 16.67 | 2.1240 | 2.1440 | 2.1340 | 2.1147 |
| 50.00 | 3.4340 | 3.3810 | 3.4075 | 3.3882 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.1 | 5.9 | 17.9 | 1.1 | 5.4 | 16.8 |
| Standard Deviation | 0.0 | 0.3 | 1.3 | 0.0 | 0.2 | 0.5 |
| Coefficient of Variation(%) | 4.1 | 5.7 | 7.0 | 3.9 | 3.1 | 2.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TNF RI/TNFRSF1A into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 128% with an overall mean recovery of 120%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.4-5.14 | 100 | 3.87 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD137/4-1BB/TNFRSF9 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: TNF R1/TNFRSF1A
Tumor necrosis factors (TNFs) are pleiotropic cytokines that are considered primary modifiers of the inflammatory and immune reactions of animals produced in response to injury or infection. Two forms of TNF, designated TNF-alpha (or cachectin) and TNF-beta (or lymphotoxin), have been described that share 30% sequence similarity and compete for binding to the same receptors. TNFs play a necessary and beneficial role as mediators of host resistance to infections and tumor formation. However, over-production or inappropriate expression of these factors can lead to a variety of pathological conditions, including wasting, systemic toxicity, and septic shock.
