Human SCF/KITLG enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.22 pg/ml (50 μl)；2.44 pg/ml (10 μl)； |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0153 | 0.0131 | 0.0142 | |
| 6.86 | 0.0424 | 0.0400 | 0.0412 | 0.0270 |
| 20.58 | 0.0919 | 0.0914 | 0.0917 | 0.0775 |
| 61.73 | 0.2355 | 0.2313 | 0.2334 | 0.2192 |
| 185.19 | 0.6456 | 0.6603 | 0.6530 | 0.6388 |
| 555.56 | 1.6390 | 1.6610 | 1.6500 | 1.6358 |
| 1666.67 | 3.0210 | 3.1300 | 3.0755 | 3.0613 |
| 5000.00 | 3.9070 | 4.0151 | 3.9611 | 3.9469 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 107.7 | 508.8 | 1485.5 | 112.3 | 503.0 | 1608.2 |
| Standard Deviation | 6.1 | 27.0 | 105.1 | 7.0 | 30.9 | 115.0 |
| Coefficient of Variation(%) | 5.6 | 5.3 | 7.1 | 6.2 | 6.1 | 7.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human SCF/KITLG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 71% to 90% with an overall mean recovery of 82%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1574.28-4366.78 | 100 | 2624.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of SCF/KITLG in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: SCF/KITLG
Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
