Human Prealbumin/Transthyretin enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.02 ng/ml (10 μl) |
| Standard Curve Range | 3.13~200 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0095 | 0.0098 | 0.0097 | |
| 3.13 | 0.0895 | 0.0823 | 0.0859 | 0.0763 |
| 6.25 | 0.1597 | 0.1545 | 0.1571 | 0.1475 |
| 12.50 | 0.3028 | 0.3084 | 0.3056 | 0.2960 |
| 25.00 | 0.6030 | 0.5964 | 0.5997 | 0.5901 |
| 50.00 | 1.1280 | 1.1260 | 1.1270 | 1.1174 |
| 100.00 | 1.9260 | 1.8610 | 1.8935 | 1.8839 |
| 200.00 | 3.0040 | 2.9850 | 2.9945 | 2.9849 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 4.2 | 19.3 | 58.0 | 4.5 | 19.9 | 59.2 |
| Standard Deviation | 0.2 | 0.9 | 2.8 | 0.1 | 0.6 | 2.4 |
| Coefficient of Variation(%) | 4.7 | 4.5 | 4.8 | 2.4 | 3.1 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Prealbumin/Transthyretin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 115% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 227.44-506.95 | 100 | 378.79 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Prealbumin/Transthyretin
Transthyretin (TTR), previously called thyroxin-binding prealbumin, is a 55 kDa homotetramer of 14-15 kDa monomers that is found in plasma. TTR is responsible for the transport of the thyroid hormone T4 and retinol binding protein (RBP). It slows renal clearance of T4 and RBP due to megalin-mediated re-uptake of TTR complexes in the renal tubules. TTR is also found in tissues including the brain. Numerous human polymorphisms have been associated with aggregates found in familial amyloidotic polyneuropathies (FAP). Mature human TTR shares 80% and 83% amino acid sequence identity with mouse and rat TTR, respectively.
