Human NKp46/NCR1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.06 pg/ml (50 μl)；1.09 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl/50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120 min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0119 | 0.0135 | 0.0127 | |
| 1.37 | 0.0232 | 0.0240 | 0.0236 | 0.0232 |
| 4.12 | 0.0501 | 0.0495 | 0.0498 | 0.0501 |
| 12.35 | 0.1274 | 0.1232 | 0.1253 | 0.1274 |
| 37.04 | 0.3634 | 0.3362 | 0.3498 | 0.3634 |
| 111.11 | 0.9253 | 0.9344 | 0.9298 | 0.9253 |
| 333.33 | 2.2430 | 2.0810 | 2.1620 | 2.2430 |
| 1000.00 | 3.7100 | 3.7730 | 3.7415 | 3.7100 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 30.2 | 162.4 | 401.4 | 22.2 | 134.6 | 462.6 |
| Standard Deviation | 1.1 | 4.7 | 6.9 | 1.3 | 4.8 | 13.7 |
| Coefficient of Variation(%) | 3.7 | 2.9 | 1.7 | 5.6 | 3.5 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human NKp46/NCR1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 109% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 115.20-331.30 | 100 | 218.98 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of NKp46/NCR1 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: NKp46/NCR1
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states. The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1, is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma. Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously. NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins.
