Human Neuropilin-1/NRP1 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.02 ng/ml (50 μl)；0.03 ng/ml (10 μl)； |
| Standard Curve Range | 0.27~200 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0061 | 0.0063 | |
| 0.27 | 0.0235 | 0.0214 | 0.0225 | 0.0162 |
| 0.82 | 0.0624 | 0.0543 | 0.0584 | 0.0521 |
| 2.47 | 0.1677 | 0.1621 | 0.1649 | 0.1587 |
| 7.41 | 0.4859 | 0.4380 | 0.4620 | 0.4557 |
| 22.22 | 1.4320 | 1.3170 | 1.3745 | 1.3683 |
| 66.67 | 3.5090 | 3.4470 | 3.4780 | 3.4718 |
| 200.00 | 4.4662 | 4.5095 | 4.4879 | 4.4816 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 7.6 | 38.8 | 148.5 | 6.5 | 33.6 | 102.7 |
| Standard Deviation | 0.3 | 1.8 | 2.7 | 0.4 | 2.0 | 2.5 |
| Coefficient of Variation(%) | 3.6 | 4.5 | 1.8 | 6.0 | 6.0 | 2.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Neuropilin-1/NRP1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 125% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 407.50-604.49 | 100 | 514.05 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Neuropilin-1/NRP1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Neuropilin-1/NRP1
Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.
