Human Nectin-4 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.15 pg/ml (50 μl)；5.23 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0126 | 0.0126 | 0.0126 | |
| 2.74 | 0.0216 | 0.0205 | 0.0211 | 0.0085 |
| 8.23 | 0.0338 | 0.0367 | 0.0353 | 0.0227 |
| 24.69 | 0.0781 | 0.0753 | 0.0767 | 0.0641 |
| 74.07 | 0.2027 | 0.2158 | 0.2093 | 0.1967 |
| 222.22 | 0.6049 | 0.6287 | 0.6168 | 0.6042 |
| 666.67 | 1.7850 | 1.8030 | 1.7940 | 1.7814 |
| 2000.00 | 3.6480 | 3.6120 | 3.6300 | 3.6174 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 49.8 | 240.7 | 861.5 | 44.4 | 211.8 | 614.5 |
| Standard Deviation | 1.9 | 11.5 | 27.1 | 1.3 | 12.3 | 35.4 |
| Coefficient of Variation(%) | 3.8 | 4.8 | 3.1 | 3.0 | 5.8 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Nectin-4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 100% to 117% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 56.23-451.61 | 100 | 226.49 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Nectin-4 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Nectin-4
Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.
