Human Myeloperoxidase enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.02 ng/ml（50 μl）；0.07 ng/ml（10 μl） |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0088 | 0.0099 | 0.0094 | |
| 0.14 | 0.0226 | 0.0236 | 0.0231 | 0.0138 |
| 0.41 | 0.0502 | 0.0532 | 0.0517 | 0.0424 |
| 1.23 | 0.1425 | 0.1269 | 0.1347 | 0.1254 |
| 3.70 | 0.4044 | 0.4130 | 0.4087 | 0.3994 |
| 11.11 | 1.1640 | 1.1530 | 1.1585 | 1.1492 |
| 33.33 | 2.9250 | 3.0390 | 2.9820 | 2.9727 |
| 100.00 | 4.4116 | 4.5568 | 4.4842 | 4.4749 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.9 | 9.1 | 34.0 | 1.8 | 9.2 | 33.6 |
| Standard Deviation | 0.1 | 0.3 | 1.6 | 0.1 | 0.3 | 0.8 |
| Coefficient of Variation(%) | 3.1 | 3.7 | 4.7 | 4.7 | 2.9 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Myeloperoxidase into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 117% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.72-19.70 | 100 | 7.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Myeloperoxidase in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Myeloperoxidase
Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.
