Human/Monkey Albumin enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.19 ng/ml (10 μl) |
| Standard Curve Range | 15.63~1000 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60 min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0424 | 0.0450 | 0.0437 | |
| 15.63 | 0.1522 | 0.1529 | 0.1526 | 0.1093 |
| 31.25 | 0.2494 | 0.2556 | 0.2525 | 0.2092 |
| 62.50 | 0.4645 | 0.4753 | 0.4699 | 0.4266 |
| 125.00 | 0.8587 | 0.8714 | 0.8651 | 0.8218 |
| 250.00 | 1.5720 | 1.5830 | 1.5775 | 1.5342 |
| 500.00 | 2.6370 | 2.7020 | 2.6695 | 2.6262 |
| 1000.00 | 3.8760 | 3.9120 | 3.8940 | 3.8507 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 22.1 | 91.2 | 315.8 | 18.3 | 97.7 | 299.5 |
| Standard Deviation | 1.1 | 4.2 | 14.2 | 0.9 | 4.2 | 12.7 |
| Coefficient of Variation(%) | 5.0 | 4.7 | 4.5 | 4.8 | 4.3 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/monkey Albumin into health human/monkey serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 107% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (mg/ml) | Detectable (%) | Mean of Detectable (mg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 7.80-30.87 | 100 | 18.32 |
| Monkey Serum | 2 | 10.66-16.21 | 100 | 13.43 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human/monkey Albumin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Albumin
Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
