Human Mesothelin enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.16 ng/ml (50 μl)；0.23 ng/ml (10 μl)； |
| Standard Curve Range | 1.37~1000 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0128 | 0.0150 | 0.0139 | |
| 1.37 | 0.0231 | 0.0235 | 0.0233 | 0.0094 |
| 4.12 | 0.0455 | 0.0442 | 0.0449 | 0.0310 |
| 12.35 | 0.1127 | 0.1187 | 0.1157 | 0.1018 |
| 37.04 | 0.3142 | 0.3075 | 0.3109 | 0.2970 |
| 111.11 | 0.8870 | 0.9468 | 0.9169 | 0.9030 |
| 333.33 | 2.5550 | 2.4420 | 2.4985 | 2.4846 |
| 1000.00 | 4.3296 | 4.4273 | 4.3785 | 4.3646 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 22.2 | 107.5 | 332.8 | 19.3 | 97.4 | 295.0 |
| Standard Deviation | 0.8 | 4.5 | 13.4 | 0.8 | 3.5 | 9.2 |
| Coefficient of Variation(%) | 3.5 | 4.2 | 4.0 | 3.9 | 3.5 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Mesothelin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 103% with an overall mean recovery of 94%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 51.87-224.14 | 100 | 119.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Mesothelin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Mesothelin
Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.
