Human MERTK enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.03 ng/ml (50 μl)；0.41 ng/ml (10 μl)； |
| Standard Curve Range | 0.27~200 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0114 | 0.0112 | 0.0113 | |
| 0.27 | 0.0191 | 0.0184 | 0.0188 | 0.0075 |
| 0.82 | 0.0343 | 0.0338 | 0.0341 | 0.0228 |
| 2.47 | 0.0751 | 0.0732 | 0.0742 | 0.0629 |
| 7.41 | 0.1987 | 0.2044 | 0.2016 | 0.1903 |
| 22.22 | 0.4883 | 0.5378 | 0.5131 | 0.5018 |
| 66.67 | 1.4530 | 1.5690 | 1.5110 | 1.4997 |
| 200.00 | 3.4070 | 3.4100 | 3.4085 | 3.3972 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 3.7 | 21.9 | 76.7 | 3.0 | 17.4 | 58.8 |
| Standard Deviation | 0.2 | 0.9 | 4.1 | 0.2 | 1.1 | 4.5 |
| Coefficient of Variation(%) | 5.5 | 4.0 | 5.3 | 5.6 | 6.1 | 7.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MERTK into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 70% to 105% with an overall mean recovery of 94%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 37.02-124.26 | 100 | 56.4 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MERTK in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: MERTK
Tyrosine-protein kinase Mer(MERTK) is a member of the TYRO3/AXL/MER (TAM) receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
