Human Lipocalin-2/NGAL enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50μl)；0.03 ng/ml (10μl)； |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0126 | 0.0115 | 0.0121 | |
| 0.14 | 0.0241 | 0.0241 | 0.0241 | 0.0121 |
| 0.41 | 0.0450 | 0.0408 | 0.0429 | 0.0309 |
| 1.23 | 0.0977 | 0.0952 | 0.0965 | 0.0844 |
| 3.70 | 0.2584 | 0.2660 | 0.2622 | 0.2502 |
| 11.11 | 0.7241 | 0.7346 | 0.7294 | 0.7173 |
| 33.33 | 1.8220 | 1.8880 | 1.8550 | 1.8430 |
| 100.00 | 3.2360 | 3.2210 | 3.2285 | 3.2165 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 2.3 | 12.0 | 41.8 | 1.7 | 9.7 | 38.1 |
| Standard Deviation | 0.1 | 0.3 | 1.8 | 0.1 | 0.5 | 1.3 |
| Coefficient of Variation(%) | 4.7 | 2.7 | 4.3 | 4.5 | 4.8 | 3.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Lipocalin-2/NGAL into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 105% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 11.47-84.79 | 100 | 49.12 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Lipocalin-2/NGAL in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Lipocalin-2/NGAL
The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.
