Human KIM-1/HAVCR1/TIM-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 pg/ml (50 μl)；2.87 pg/ml (10 μl)； |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0108 | 0.0102 | 0.0105 | |
| 1.37 | 0.0178 | 0.0167 | 0.0173 | 0.0068 |
| 4.12 | 0.0284 | 0.0298 | 0.0291 | 0.0186 |
| 12.35 | 0.0646 | 0.0667 | 0.0657 | 0.0552 |
| 37.04 | 0.1792 | 0.1831 | 0.1812 | 0.1707 |
| 111.11 | 0.4724 | 0.4993 | 0.4859 | 0.4754 |
| 333.33 | 1.3460 | 1.3870 | 1.3665 | 1.3560 |
| 1000.00 | 3.3360 | 3.3650 | 3.3505 | 3.3400 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 14.1 | 103.9 | 401.6 | 16.1 | 102.7 | 314.1 |
| Standard Deviation | 1.0 | 6.9 | 30.6 | 0.7 | 2.9 | 14.4 |
| Coefficient of Variation(%) | 7.1 | 6.6 | 7.6 | 4.1 | 2.9 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human KIM-1/HAVCR1/TIM-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 125% with an overall mean recovery of 111%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 7.08-28.75 | 100 | 14.61 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: KIM-1/HAVCR1/TIM-1
TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain. The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing. For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice. This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented. In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule. The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin. TIM-1 ligation induces T cell proliferation and promotes cytokine production. In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif.
