Human IL1RL1/DER4/ST2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.59 pg/ml (50 μl)；8.53 pg/ml (10 μl)； |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl/50 μl |
| Type | Ready-to-Use |
| Operation Duration | 120 min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0084 | 0.0073 | 0.0079 | |
| 13.72 | 0.0170 | 0.0168 | 0.0169 | 0.0091 |
| 41.15 | 0.0341 | 0.0368 | 0.0355 | 0.0276 |
| 123.46 | 0.0847 | 0.0891 | 0.0869 | 0.0791 |
| 370.37 | 0.2346 | 0.2441 | 0.2394 | 0.2315 |
| 1111.11 | 0.5879 | 0.5783 | 0.5831 | 0.5753 |
| 3333.33 | 1.5660 | 1.5200 | 1.5430 | 1.5352 |
| 10000.00 | 3.1700 | 3.1730 | 3.1715 | 3.1637 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 211.53 | 980.92 | 2967.31 | 245.35 | 1173.26 | 3093.95 |
| Standard Deviation | 9.43 | 46.15 | 168.39 | 17.87 | 72.26 | 172.49 |
| Coefficient of Variation(%) | 4.5 | 4.7 | 5.7 | 7.3 | 6.2 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL1RL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 120% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 17.61-61.57 | 100.0 | 28.48 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL1RL1 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL1RL1/DER4/ST2
ST2, also known as IL-1R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.
