Human IL-18 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.56 pg/ml (50 μl)；1.28 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Full-Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0449 | 0.0488 | 0.0469 | |
| 6.86 | 0.0752 | 0.0687 | 0.0720 | 0.0251 |
| 20.58 | 0.1038 | 0.0908 | 0.0973 | 0.0505 |
| 61.73 | 0.1749 | 0.1668 | 0.1709 | 0.1240 |
| 185.19 | 0.4421 | 0.4094 | 0.4258 | 0.3789 |
| 555.56 | 1.1370 | 1.1090 | 1.1230 | 1.0762 |
| 1666.67 | 2.7832 | 2.6478 | 2.7155 | 2.6687 |
| 5000.00 | 4.2824 | 4.3490 | 4.3157 | 4.2689 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 99.5 | 490.4 | 1512.9 | 101.2 | 481.1 | 1476.2 |
| Standard Deviation | 5.0 | 32.7 | 74.9 | 3.7 | 22.8 | 86.3 |
| Coefficient of Variation(%) | 5.1 | 6.7 | 4.9 | 3.6 | 4.7 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-18 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 111% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 249.34- 3143.95 | 100 | 1382.96 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-18 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-18
Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1 gamma, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-gamma and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-gamma that inhibits IgE production.
