Human IL-17F enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.60 pg/ml (50 μl)；1.17 pg/ml (10 μl)； |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0201 | 0.0188 | 0.0188 | |
| 1.37 | 0.0255 | 0.0254 | 0.0255 | 0.0067 |
| 4.12 | 0.0396 | 0.0393 | 0.0395 | 0.0207 |
| 12.35 | 0.0677 | 0.0694 | 0.0686 | 0.0498 |
| 37.04 | 0.1612 | 0.1615 | 0.1614 | 0.1426 |
| 111.11 | 0.4124 | 0.4180 | 0.4152 | 0.3964 |
| 333.33 | 1.0830 | 1.2010 | 1.1420 | 1.1232 |
| 1000.00 | 2.6840 | 2.8360 | 2.7600 | 2.7412 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 15.0 | 88.2 | 294.2 | 18.8 | 98.7 | 302.5 |
| Standard Deviation | 1.2 | 6.9 | 18.9 | 0.5 | 4.7 | 3.5 |
| Coefficient of Variation(%) | 7.8 | 7.8 | 6.4 | 2.7 | 4.8 | 1.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-17F into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 102% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-13.94 | 25 | 4.76 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-17F
IL-17F is a disulfide-linked dimeric cytokine expressed in activated CD4+ T-cells and activated monocytes. It signals through IL-17 RC to stimulate production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis. IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells.
