Human IL-17E/IL-25 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.69 pg/ml (50 μl)；2.74 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0112 | 0.0117 | 0.0115 | |
| 2.74 | 0.0180 | 0.0179 | 0.0180 | 0.0065 |
| 8.23 | 0.0321 | 0.0308 | 0.0315 | 0.0200 |
| 24.69 | 0.0710 | 0.0689 | 0.0700 | 0.0585 |
| 74.07 | 0.2000 | 0.1808 | 0.1904 | 0.1790 |
| 222.22 | 0.5739 | 0.5533 | 0.5636 | 0.5522 |
| 666.67 | 1.6250 | 1.4920 | 1.5585 | 1.5471 |
| 2000.00 | 3.6720 | 3.5570 | 3.6145 | 3.6031 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 40.3 | 180.8 | 566.4 | 38.9 | 186.5 | 589.6 |
| Standard Deviation | 2.0 | 10.2 | 57.1 | 2.3 | 8.8 | 35.4 |
| Coefficient of Variation(%) | 4.9 | 5.7 | 10.1 | 5.8 | 4.7 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-17E/IL-25 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 117% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-4.62 | 20 | 2.30 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-17E/IL-25 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-17E
The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
